Two-temperature membrane filter method for enumerating fecal coliform bacteria from chlorinated effluents
نویسندگان
چکیده
منابع مشابه
Membrane filter method for recovery of fecal coliforms in chlorinated sewage effluents.
The standard one-step M-FC broth-membrane-filter procedure for recovery of fecal coliforms from chlorinated sewage effluents is much less effective than the multiple-tube (most-probable-number) technique. A two-step membrane-filter method, using a pre-enrichment technique with phenol red lactose broth and incubation at 35 degrees C for 4 h, followether 18+/-2 h, enhanced fecal coliform recovery...
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A membrane filter procedure for enumerating Escherichia coli was developed and evaluated. The method quantifies E. coli within 24 h without requiring subculture and identification of isolates. It incorporates a primary selective-differential medium for gram-negative, lactose-fermenting bacteria; resuscitation of weakened organisms by incubation for 2 h at 35 degrees C before incubation at 44.5 ...
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The accepted method for determining disinfection efficiency of wastewater treatment systems is routinely to perform fecal coliform bacteria analyses on the final effluent (5). Standard Methods for the Examination of Water and Wastewater outlines two procedures for fecal coliform analysis: the membrane filtration technique and the most-probable-number (MPN) method (1). For chlorinated effluents,...
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A facile, quantitative, membrane filter procedure (mC) for defining the distribution of coliform populations in seawater according to the component genera was developed. The procedure, which utilizes a series of in situ substrate tests to obviate the picking of colonies for identification, also provides an estimate of the total coliform density. When pure cultures of Escherichia coli, Klebsiell...
متن کاملRapid, single-step most-probable-number method for enumerating fecal coliforms in effluents from sewage treatment plants.
A single-step most-probable-number method for enumerating fecal coliforms in sewage treatment plant effluents is described. The method requires the use of only one lactose-based medium and a single incubation temperature of 44.5 degrees C, and it can be completed in 18 h or less, as compared with up to 72 h for the standard most-probable-number method. The appearance of growth is the sole crite...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1977
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.33.6.1259-1264.1977